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Table 1 Acinetobacter baylyi strains used in this study

From: Removal of aromatic inhibitors produced from lignocellulosic hydrolysates by Acinetobacter baylyi ADP1 with formation of ethanol by Kluyveromyces marxianus

Strain

Relevant characteristics

Sources

ADP1

Wild type (BD413)

Juni and Janik [19]

ACN462

ΔpcaHG5462

Deletion of DNA from 1,717,153 to 1,715,814a replaced with GCGGCCGC, the recognition sequence for NotI

PCR fragment X ADP1; screened for loss of ability to grow on 4HB c

[7]

ACN472

ΔbenD5472

Deletion of DNA from 1,437,252 to 1,438,007a replaced with GCGGCCGC, the recognition sequence for NotI

PCR fragment X ADP1; screened for loss of ability to grow on benzoate b

Eby [7]

ACN2069

Δgcd::sacB-KmR52069

pBAC1566/AatII X ADP1; selected by Km R

This study

ACN2070

Δgcd::sacB-KmR52069; ΔpcaHG5462

pBAC1566/AatII X ACN462; selected by Km R

This study

ACN2071

Δgcd::sacB-KmR52069; ΔbenD5472

pBAC1566/AatII X ACN472; selected by Km R

This study

ACN2089

Δgcd52089

pBAC1565/AatII X ACN2069; selected by growth in the presence of sucrose b

This study

ACN2090

Δgcd52089; ΔpcaHG5462

pBAC1565/AatII X ACN2070; selected by growth in the presence of sucrose b

This study

ACN2091

Δgcd52089; ΔbenD5472

pBAC1565/AatII X ACN2071; selected by growth in the presence of sucrose b

This study

  1. Strains were derived from ADP1, previously known as Acinetobacter calcoaceticus or Acinetobacter sp. [53]
  2. aItalics numbers correspond to positions on the ADP1 chromosome in NCBI entry NC_005966
  3. bUnderlined text indicates the donor DNA and, where relevant, the restriction enzyme used to linearize a plasmid (pBAC number/enzyme). The donor DNA was used to transform (X) the indicated recipient strain