Fig. 2

Overview of gene expression among yeast strains engineered for increased lipid production and cellular lipid content. a Multidimensional scaling (MDS) plot of gene expressions among different strains, b variance percentage by MDS of different dimensions. The MDS plot was generated by the Degust program, which was a means of visualizing the similar gene expression level of individual strain of the RNA-seq data set. The between-strain distance in the MDS plot determined the similarity of overall gene expression between two H-series strains. c Number of differentially expressed genes (DEG, including up- and downregulated) between engineered strains and control, d venn diagram of DEG between selected engineered strains H14, 20, 27, and 31. e Comparison of relative lipid content between four target strains using Nile red staining by flow cytometry, the lipid content of control was normalized to 1. *p < 0.05, **p < 0.01 (Student’s t test: two-tailed, two-sample equal variance)