Fig. 3From: Combining directed evolution of pathway enzymes and dynamic pathway regulation using a quorum-sensing circuit to improve the production of 4-hydroxyphenylacetic acid in Escherichia coli4HPAA production by E. coli DOPA-30N harboring the fusion gene cluster of different combinations of the evolved S. cerevisiae ARO10 and E. coli feaB. Growth (white bar); 4HPAA concentration (gray bar). Cells were grown at 30 °C and 200 rpm for 72 h. See “Methods” for other details. The data represent the means of three replicates, and error bars represent standard deviationsBack to article page