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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Combining directed evolution of pathway enzymes and dynamic pathway regulation using a quorum-sensing circuit to improve the production of 4-hydroxyphenylacetic acid in Escherichia coli

Fig. 5

Growth (white bar) and 4HPAA production (gray bar) for E. coli DOPA-30N harboring the TIGR-mediated gene cluster of the evolved S. cerevisiae ARO10* and E. coli feaB*. a Colonies with lighter colors from the TIGR library; b strains with higher levels of 4HPAA. Cells were grown at 30 °C and 200 rpm for 72 h. See “Methods” for other details. E. coli DOPA-30N (pBbB2k-ARO10*-L-feaB*) was set the control. The data represent the means of three replicates, and error bars represent standard deviations

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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