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Table 2 4-Hydroxyphenylacetic acid production in E. coli DOPA-30N harboring different biosynthetic pathways

From: Combining directed evolution of pathway enzymes and dynamic pathway regulation using a quorum-sensing circuit to improve the production of 4-hydroxyphenylacetic acid in Escherichia coli

Host strain

Plasmid

OD600

Titer (g/L)

Yield (%, mol/mol)

E. coli DOPA-30N

pBbB2k-ipdC-L-feaB

16.28 ± 0.45

2.32 ± 0.05

11.8 ± 0.2

pBbB2k-ARO10-L-feaB

17.36 ± 0.19

3.08 ± 0.05

13.4 ± 0.2

pBbB2k-aas-L-feaB

17.13 ± 1.44

2.11 ± 0.06

9.3 ± 0.2

pBbB2k-tydC-L-tynA-L-feaB

15.44 ± 0.42

2.41 ± 0.04

12.1 ± 0.2

pBbB2k-ARO10*-L-feaB*

16.68 ± 0.35

5.64 ± 0.06

22.9 ± 0.3

pBbB2k-ARO10*-TIGR-feaB*

17.12 ± 0.32

6.58 ± 0.16

27.5 ± 0.3

  1. Data represent the means of three replicates and standard deviations
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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