Fig. 1

Effects of surfactant supplementation and shaking on conversions of cellulosic substrates at low enzyme loading. Cellulose conversions are reported after 5, 11, and 17 days of enzymatic hydrolysis of model cellulosic substrates at 1% glucan loadings using 5 mg cellulase protein (Accellerase^® 1500) per g glucan in shaken flasks, 5 mg Tween 20 per g glucan-supplemented shaken flasks, and unshaken flasks, all with a 50 mL reaction volume in 125 mL Erlenmeyer flasks. S shaking, NS no shaking. Data for Avicel conversions are from Bhagia et al. [14]