Fig. 6

EMSA analysis of the interactions between the DNA-binding domain PoxCxrA_17–150 and the promoter regions of genes encoding putative sugar transporters. Each EMSA experiment comprised PoxCxrA_17–150 (0–2 µg) and 50 ng of target probes labelled with 6-carboxyfluorescein (6-FAM). Probes without 6-FAM, and the promoter of β-tubulin gene POX05989, were used as competitive probes and negative control, respectively. The fusion protein Trx–His–S purified from E. coli cells harbouring the empty plasmid pET32a (+), and BSA alone, were used as protein controls