Fig. 7
The PoxCxrA17–150-binding sequence in the promoter region of cellobiohydrolase gene POX05587/Cel7A-2. a DNase I footprinting experiment results. b EMSA confirmation of PBM_POX05587. c Schematic diagram of PBM_POX05587 truncation. d Key nucleotides in PBM_POX05587 confirmed via EMSA experiments. Each EMSA experiment comprised 2 µg of PoxCxrA17–150 and 50 ng of truncated PBM_POX05587 as probe labelled with 6-carboxyfluorescein. The promoter of β-tubulin gene POX05989 was used as a probe control. The fusion protein Trx–His–S purified from E. coli cells harbouring the empty plasmid pET32a (+), and BSA alone, were used as protein controls. ‘−’ and ‘+’ represent EMSA experiments without and with DBD PoxCxrA17–150, respectively