Fig. 6From: Differential transcriptomic profiling of filamentous fungus during solid-state and submerged fermentation and identification of an essential regulatory gene PoxMBF1 that directly regulated cellulase and xylanase gene expressionIn vitro binding experiments using recombinant protein rPoxMBF1 and the promoter regions of target genes. Each EMSA reaction system contained 0–2.0 μg of Trx–His–S-tagged rPoxMBF1 and ~ 40 ng of each candidate probe. The same amounts of purified Trx–His–S fusion protein, BSA, and ITS sequence were, respectively, employed as negative controlsBack to article page