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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana

Fig. 1

Expression analysis of CCoAOMT1 and FPGS gene family members in different organs of wild-type (WT) Arabidopsis plants by qRT-PCR. Relative transcript levels of CCoAOMT1 are presented on the left y axis and FPGS gene family are presented on the right y axis. The labels for both sides are of different scales. Root and shoot tissues were from 2-week-old seedlings; Stem, leaf (youngest leaves), and flower (opened flowers) tissues were from 6-week-old plants; Stem-Top: top parts of stem above 1st silique; Stem-Middle: stem tissues between 1st branch and 1st silique; Stem-Bottom: stem tissues below the 1st branch; The AtACTIN2 (AT3G18780) was used as the reference gene. Relative fold changes were obtained by qRT-PCR using the 2−ΔΔCT method. Values are means ± SD from three biological replicates with three technical replicates per biological replicate. Each biological replicate contained 20 plants. Different letters indicate statistically significant differences between values according to one-way ANOVA and LSD test (P ≤ 0.05)

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