Fig. 4

Glycome-profiling heatmaps of sequential extracts by increasingly harsher reagents from alcohol-insoluble (cell wall) residues (AIR) prepared from 6-week-old Arabidopsis stems of WT, fpgs1, ccoaomt1, and fpgs1ccoaomt1 plants. The top bar graph showed the total carbohydrates extracted from each reagent (from ammonium oxalate, sodium carbonate, 1 M KOH, 4 M KOH, acidic sodium chlorite, to 4 M KOH PC). The heatmap indicated the strength of different monoclonal antibodies (mAbs) binding with the non-cellulosic cell-wall components extracted from each reagent. Bright yellow showed the strongest binding, dark blue for no binding