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Table 1 Total sugar release, enzymatic sugar release, and enzymatic hydrolysis efficiencies in 6-week-old stems of WT, fpgs1, ccoaomt1, and fpgs1ccoaomt1 plants

From: Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana

Line name Total sugar release (mg/g AIR) Enzymatic sugar release (mg/g AIR) Enzymatic hydrolysis efficiencies (%)
WT 530.1 ± 29.1c 122.5 ± 15.5c 21.8 ± 2.6c
fpgs1 585.9 ± 26.7b 136.6 ± 9.1bc 22.9 ± 3.2bc
ccoaomt1 605.3 ± 11.5ab 145.1 ± 26.4b 24.0 ± 3.5b
fpgs1ccoaomt1 619.7 ± 21.0a 169.9 ± 17.5a 27.7 ± 4.1a
  1. Total sugar release (TSR) and enzymatic sugar release (ESR) were estimated from destarched alcohol-insoluble residues (AIR) of stem tissues from 6-week-old Arabidopsis plants without acid pretreatment. Enzymatic hydrolysis efficiency is expressed as a percentage of the ESR yield to the TSR yield for each replicate prior to obtain average values. Each value represents six biological replicates and each biological replicate contained 20 plants. Different letters in each column indicate statistically significant differences between values in the columns according to one-way ANOVA and LSD test (values are mean ± SE, n = 6, P ≤ 0.05)