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Table 1 Total sugar release, enzymatic sugar release, and enzymatic hydrolysis efficiencies in 6-week-old stems of WT, fpgs1, ccoaomt1, and fpgs1ccoaomt1 plants

From: Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana

Line name

Total sugar release (mg/g AIR)

Enzymatic sugar release (mg/g AIR)

Enzymatic hydrolysis efficiencies (%)


530.1 ± 29.1c

122.5 ± 15.5c

21.8 ± 2.6c


585.9 ± 26.7b

136.6 ± 9.1bc

22.9 ± 3.2bc


605.3 ± 11.5ab

145.1 ± 26.4b

24.0 ± 3.5b


619.7 ± 21.0a

169.9 ± 17.5a

27.7 ± 4.1a

  1. Total sugar release (TSR) and enzymatic sugar release (ESR) were estimated from destarched alcohol-insoluble residues (AIR) of stem tissues from 6-week-old Arabidopsis plants without acid pretreatment. Enzymatic hydrolysis efficiency is expressed as a percentage of the ESR yield to the TSR yield for each replicate prior to obtain average values. Each value represents six biological replicates and each biological replicate contained 20 plants. Different letters in each column indicate statistically significant differences between values in the columns according to one-way ANOVA and LSD test (values are mean ± SE, n = 6, P ≤ 0.05)