Fig. 2

Cell-wall characterization and digestibility of sugarcane straw biomass. a Saccharification of sugarcane straw from SacBAHD01 RNAi lines and nontransformed (NT) plants after Organosolv pretreatment, using Cellic CTec 3 (Novozymes) at 15 FPU for 6 and 48 h. b Ester-linked contents of ferulate (FA) and p-coumarate acid (pCA) in the alcohol insoluble fraction (AIR) of sugarcane straw from three independent events of SacBAHD01 RNAi lines and NT plants. c HCA conjugates in supernatant following mild acidolysis of sugarcane straw AIR. The data are represented as relative peak areas of major peaks for p-coumarate (pCA)-Ara and ferulate (FA)-Ara, previously identified by LC–MS as described by de Souza et al. [1]. n = 4; error bars ± SEM; significance of difference of transgenic lines from NT plants indicated if difference in means > least significant difference from ANOVA at p < 0.01 (*) and p < 0.001 (**)