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Table 1 Real and coded levels of variables used in CCD experiments and the responses glucose concentrations obtained with 6- and 48-h enzymatic hydrolysis ± SD

From: Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility

Trials X1
Ethanol concentration (% v/v)
X2
Residence time (min)
NT Ev. 1 Ev. 2.2 Ev. 2.4 Ev. 3.1 Ev. 4
Glucose 6 h (g L−1)
 1 − 1.0 (40.2) − 1.0 (34.9) 13.48 ± 0.16b 13.30 ± 0.66b 14.06 ± 0.82ab 15.11 ± 0.11a 14.75 ± 0.26a 11.95 ± 0.32c
 2 − 1.4 (30.0) + 1.0 (205.1) 21.75 ± 1.08a 21.04 ± 0.76ab 20.12 ± 0.05ab 20.27 ± 0.25ab 19.37 ± 0.70ab 18.79 ± 0.86b
 3 + 1.0 (89.8) − 1.0 (34.9) 7.91 ± 0.15ab 7.37 ± 0.28b 8.30 ± 0.35ab 8.24 ± 0.32a 8.03 ± 0.50ab 7.43 ± 0.22b
 4 + 1.0 (89.8) + 1.0 (205.1) 10.15 ± 0.37a 9.94 ± 0.28a 10.15 ± 0.31a 10.53 ± 0.44a 10.18 ± 0.43a 9.77 ± 0.18a
 5 − 1.4 (30.0) 0 (120.0) 21.08 ± 0.46ab 21.84 ± 0.87a 21.35 ± 0.62ab 20.05 ± 0.71b 21.05 ± 0.92ab 19.86 ± 0.41b
 6 + 1.4 (100.0) 0 (120.0) 6.26 ± 0.13c 6.19 ± 0.18c 7.20 ± 0.30a 6.95 ± 0.36ab 6.49 ± 0.10bc 6.95 ± 0.06ab
 7 0 (65.0) − 1.4 (0.0) 7.44 ± 0.30bc 7.42 ± 0.15bc 8.28 ± 0.49a 8.00 ± 0.33ab 7.61 ± 0.19abc 7.12 ± 0.29c
 8 0 (65.0) + 1.4 (240.0) 16.89 ± 0.57ab 16.96 ± 1.04ab 15.44 ± 0.56b 17.93 ± 1.06a 17.94 ± 0.58a 16.47 ± 0.34ab
 9 0 (65.0) 0 (120.0) 16.22 ± 0.67a 15.56 ± 0.35a 15.11 ± 0.73a 16.18 ± 0.90a 15.36 ± 0.87a 16.12 ± 0.79a
 10 0 (65.0) 0 (120.0) 13.02 ± 0.37bc 13.15 ± 0.23bc 13.58 ± 0.23ab 14.62 ± 0.65a 12.28 ± 0.09 cd 12.05 ± 0.58d
 11 0 (65.0) 0 (120.0) 10.95 ± 0.51b 12.65 ± 0.12a 10.56 ± 0.47b 13.56 ± 0.68a 12.62 ± 0.29a 10.76 ± 0.58b
Glucose 48 h (g L−1)
 1 − 1.0 (40.2) − 1.0 (34.9) 23.07 ± 0.46b 20.42 ± 0.77bc 22.07 ± 0.38bc 26.85 ± 1.56a 26.62 ± 2.80a 19.68 ± 1.24c
 2 − 1.4 (30.0) + 1.0 (205.1) 31.33 ± 0.86c 35.68 ± 2.58a 32.31 ± 0.55b 34.74 ± 0.75a 31.75 ± 0.04c 28.46 ± 1.45d
 3 + 1.0 (89.8) − 1.0 (34.9) 8.92 ± 0.66b 9.09 ± 0.66b 10.65 ± 0.40a 9.53 ± 1.01ab 10.03 ± 0.75ab 9.29 ± 0.28ab
 4 + 1.0 (89.8) + 1.0 (205.1) 13.69 ± 1.38a 13.07 ± 0.39a 14.72 ± 1.19a 13.95 ± 0.56a 14.66 ± 1.00a 13.37 ± 0.88a
 5 − 1.4 (30.0) 0 (120.0) 31.77 ± 0.65bc 38.00 ± 0.96a 33.93 ± 1.53b 33.49 ± 1.59b 29.62 ± 0.76c 31.74 ± 0.88bc
 6 + 1.4 (100.0) 0 (120.0) 8.49 ± 0.28a 8.51 ± 0.17a 8.93 ± 0.24a 9.31 ± 0.44a 8.63 ± 0.49a 8.44 ± 1.13a
 7 0 (65.0) − 1.4 (0.0) 8.74 ± 0.29c 8.74 ± 0.06c 10.28 ± 0.85a 9.94 ± 0.31ab 9.17 ± 0.10bc 8.76 ± 0.71c
 8 0 (65.0) + 1.4 (240.0) 26.27 ± 0.23ab 29.33 ± 3.55a 22.39 ± 0.44b 29.01 ± 2.81a 28.71 ± 3.22a 24.97 ± 1.00ab
 9 0 (65.0) 0 (120.0) 25.75 ± 2.74a 22.29 ± 2.23a 22.01 ± 2.71a 22.67 ± 1.63a 22.45 ± 3.09a 22.65 ± 4.00a
 10 0 (65.0) 0 (120.0) 25.17 ± 0.28a 23.02 ± 1.78ab 24.23 ± 0.76a 24.89 ± 2.11a 22.66 ± 1.30ab 20.14 ± 0.44b
 11 0 (65.0) 0 (120.0) 16.89 ± 1.53c 25.51 ± 0.63a 16.02 ± 0.87c 22.97 ± 0.88b 23.26 ± 1.39ab 15.57 ± 1.13c
  1. Means followed by the same lower case letters in a line do not differ significantly by the Tukey test (p < 0.05). In this case, letters should be compared only between the rows, but not between different trials. For each experimental condition, presented in a table row, a Tukey test was performed. These results are from four independent replicates. Ev. refers to different transgenic events. NT: nontransformed plants