Development of an oligosaccharide library to characterise the structural variation in glucuronoarabinoxylan in the cell walls of vegetative tissues in grasses

Table 2 Enzymatic analysis of GAX oligosaccharides

Oligosaccharide	Unknown structure	GH10 product	GH11 product	Enzyme sensitivity
Oligosaccharide	Unknown structure	GH10 product	GH11 product	CgGH3 xylosidase	PcGH51 α-arabinosidase	PaGH62 α-arabinosidase	CjGH67 α-glucuronidase	BoGH115 α-glucuronidase
U^(4Me)2X	N₁	●●	●	−	−	−	−	+
U²XX	N₂	●		−	−	−	+	+
U^(4Me)2XX	N₃	●●	●	−	−	−	+	+
A³X	N₄	●●		−	+	+	NT	−
XU ² XX	−		●	−	−	−	+	+
XU ^(4Me)2 XX	−		●	−	−	−	+	+
XA³X	N₅	●●	●●	NT	+	+	NT	−
A³A³X	N₆ (Z₁)	●		NT	+	−	NT	−
A³XXX	N₇ (Z₂)	●		NT	+	+	NT	NT
XA ³ XX	−		●●	−	NT	+	NT	−
D^2,3XX	N₈ (Z₃)	●		+	−	−	NT	−
XA³A³X	N₉	●		−	+	−	NT	NT
A³U^(4Me)2XX	N₁₀		●	NT	+	−	NT	−
B^2,3U^(4Me)2XX or D^2,3U^(4Me)2XX	N₁₁		●	−	−	NT	NT	−
XA³XU^(4Me)2XX	N₁₂		●	NT	+	+	NT	+
D^2,3X	M₁	●		+	−	−	NT	−
XD^2,3XX	M₂		●	+	−	−	NT	−

The dots indicate, which xylanase produces the oligosaccharide; one dot (●) indicates minor products while two dots (●●) indicate major products. Oligosaccharides sensitive to GH enzymes are marked with a plus (+), those resistant with a minus (−); NT not tested. Oligosaccharides XU²XX, X^(4Me)2XX and XA³XX have been previously structurally characterised [27,28,29]

ISSN: 2731-3654