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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase

Fig. 2

Biochemical characterization of TON-LPL and rc-TGL. Relative activities at different temperatures and pH are shown in a, b for TON-LPL (black) and rc-TGL (gray), respectively. All reactions were incubated for 5 min and performed in triplicate with pNP-butyrate. For different pH, the incubation temperature for TON-LPL and rc-TGL was 70 °C and 60 °C, respectively. The specific activity with different substrates; pNP C-2 (acetate); pNP C-4 (butyrate); pNP C-8 (octanoate), pNP C-12 (dodecanoate) and pNP-C-16 (palmitate) is shown in c for TON-LPL (black) and rc-TGL (gray). All assays with triglycerides were performed in triplicates and the enzymatic activity was determined by the titration method and shown in rc-TGL (gray) and TLIP (light black). The specific activity with different triglyceride substrates; Tri-C2, Tri-C4, castor oil, and olive oil is shown in d. All assays were performed in triplicates

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