Fig. 3From: N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipaseThermal inactivation kinetics of TON-LPL (black) and rc-TGL (gray). TON-LPL and rc-TGL were incubated at 60 °C and 70 °C in a 100 mM phosphate buffer of pH 7.0 (a and b, respectively). At different time intervals, the aliquots were taken and followed by an assay with pNP-butyrate as described in method section. All assays were performed in triplicates, and relative activities are shownBack to article page