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Table 3 Effects of the three major biomass inhibitors were acetic acid (X1), formic acid (X2), and (X3) (levulinic acid) added to the SD-medium on the yields of ethanol yields and biomass by applying the 23 full-central-composite design

From: Statistical prediction of interactions between low concentrations of inhibitors on yeast cells responses added to the SD-medium at low pH values

Runs X 1 X 2 X 3 Biomass (mg/mL) Ethanol (g/L)
Observed values Predicted
values
Observed values Predicted values
1 5.0 25.0 10.0 12.4 ± 0.6 12.4 61.1 ± 1.5 60.7
2 10.0 25.0 10.0 11.6 ± 0.6 11.6 49.8 ± 4.9 51.9
3 5.0 35.0 10.0 11.8 ± 0.1 11.9 51.2 ± 2.2 49.4
4 10.0 35.0 10.0 11.2 ± 0.3 11.2 33.1 ± 3.6 37.2
5 5.0 25.0 20.0 12.2 ± 0.5 12.2 44.3 ± 2.5 41.4
6 10.0 25.0 20.0 11.2 ± 0.8 11.1 31.9 ± 2.8 34.7
7 5.0 35.0 20.0 11.3 ± 0.1 11.3 41.9 ± 5.3 40.8
8 10.0 35.0 20.0 10.3 ± 0.1 10.6 29.1 ± 2.6 30.7
9 3.3* 30.0 15.0 12.7 ± 0.7 12.6 46.7 ± 2.5 50.9
10 11.7* 30.0 15.0 11.1 ± 0.5 11.1 40.9 ± 4.1 35.1
11 7.5 21.6* 15.0 11.9 ± 0.5 11.9 52.1 ± 1.7 51.7
12 7.5 38.4* 15.0 11.0 ± 0.4 10.9 39.9 ± 2.3 38.8
13 7.5 30.0 6.6* 11.7 ± 0.8 11.7 52.8 ± 3.3 50.9
14 7.5 30.0 23.4* 10.8 ± 0.2 10.8 28.9 ± 1.9 29.2
15 7.5 30.0 15.0 10.7 ± 0.2 11.0 37.0 ± 2.2 33.2
16 7.5 30.0 15.0 11.2 ± 0.5 11.0 34.2 ± 1.4 33.2
17 7.5 30.0 15.0 11.2 ± 0.2 11.0 32.7 ± 1.0 33.2
18 7.5 30.0 15.0 10.6 ± 0.3 11.0 32.4 ± 1.1 33.2
19 7.5 30.0 15.0 11.5 ± 0.7 11.0 29.2 ± 0.4 33.2
20 7.5 30.0 15.0 11.0 ± 0.1 11.0 33.1 ± 0.1 33.2
  1. Fermentations were performed using strain the yeast strain 63 M and the inhibitors (mmol/L) were as follows: X1 (acetic acid), X2 (formic acid), and X3 (levulinic acid), while the stars (*) indicates Axial points