Fig. 2

TLC analysis of hydrolysis products from beechwood xylan by recombinant TtXyn30A. The reaction was carried out in 0.05 mM citrate–phosphate buffer pH 4.0 at 50 °C. The substrate and enzyme loadings were 5 mg mL⁻¹ and 0.09 U mL⁻¹, respectively. Xylooligosaccharides (DP 2–6) and aldouronic acids (aldotetrauronic and aldopentauronic acid) were used as standards