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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Fig. 1

Schematic drawings of chloroplast and cER localization in Nannochloropsis, and vector construction. a Simplified cellular structure of Nannochloropsis including organelles, in particular, the chloroplast and the surrounding cER. Therefore, chloroplast localization of proteins requires signals for the signal peptide for cER (SP-ER) and the chloroplast transit peptide (cTP). b Construction of the pNsChlb vector for expression of the catalytic domain of CrCAO cloned from Chlamydomonas reinhardtii. For proper localization of CAO to the chloroplast, signal peptide and cTP from the GCSL gene from N. salina were added to CrCAO. The recombinant CrCAO was tagged with the Flag tag, and the vector contained the Shble marker

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