Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Table 1 Photosynthetic pigments analyzed by HPLC

(Scale: μg/mg)	Pigments	ML (90 μE)			HL (200 μE)
(Scale: μg/mg)	Pigments	WT	NsChlb 7	NsChlb 19	WT	NsChlb 7	NsChlb 19
Day 8	Chla	45.36 ± 3.13	57.61 ± 1.69*	50.09 ± 1.09	43.92 ± 3.42	50.70 ± 2.30	39.48 ± 2.44
	Chlb	n.d.	0.28 ± 0.05	1.51 ± 0.13	n.d.	0.18 + 0.01	0.53 + 0.07
	Violaxanthin	3.88 ± 0.06	4.48 ± 0.09	4.04 ± 0.17	4.10 ± 0.29	5.06 ± 0.26*	3.90 ± 0.21
	β-carotene	4.42 ± 0.45	4.07 ± 0.83	4.58 ± 0.93	1.08 ± 0.42	2.36 ± 0.06**	1.85 ± 0.07*
	Zeaxanthin	0.09 ± 0.01	0.12 ± 0.01	0.16 ± 0.01	0.10 ± 0.01	0.11 ± 0.01	0.10 ± 0.01
Day 12	Chla	72.52 ± 1.90	75.86 ± 8.84	70.55 ± 4.43	23.25 ± 1.30	25.86 ± 1.16	20.23 ± 1.30
	Chlb	n.d.	0.15 ± 0.07	0.97 ± 0.40	n.d.	0.05 ± 0.02	0.13 ± 0.05
	Violaxanthin	7.37 ± 0.08	7.52 ± 1.22	7.42 ± 0.29	1.81 ± 0.19	2.10 ± 0.23	1.70 ± 0.17
	β-carotene	5.68 ± 0.09	5.84 ± 0.55	5.49 ± 0.19	1.39 ± 0.10	1.67 ± 0.17	1.34 ± 0.02
	Zeaxanthin	0.12 ± 0.01	0.11 ± 0.01	0.09 ± 0.20	0.17 ± 0.01	0.20 ± 0.03	0.13

The data represent the average value with ranges of standard error (n = 3). Significant differences between WT and each transformants (WT vs. NsChlb7, WT vs. NsChlb19) are determined by Student’s t test and are indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001)

ISSN: 2731-3654