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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction

Fig. 3

Demonstration of the tobacco leaf sgRNA efficiency assay. Each side of a tobacco leaf was infiltrated with one test construct. GFP and RFP fluorescence images were captured sequentially for the same leaf. The tests for a weak sgRNA (HCT_gRNA8) and a strong sgRNA (HCT_gRNA9) with three biological replicates are shown as Leafs 1, 2, and 3. Representative areas (red circles) on leaf were selected and measured for integrated intensity using ImageJ software. The ratio of GFP intensity vs. RFP intensity of the three representative areas was averaged for each biological replicate. The editing efficiency of each sgRNA was evaluated based on GFP:RFP ratio obtained from 5–7 biological replicates for each assay

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