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Table 4 Setup for the different phases of the continuous formation of valerate from propionate and methanol to study the effect of pH lowering on n-valerate formation

From: Continuous n-valerate formation from propionate and methanol in an anaerobic chain elongation open-culture bioreactor

Phase conditions

Phase I

Phase II

Phase III

Phase IV

Hydraulic retention time (h)

42.3

95.2

95.2

95.2

Phase duration (days)

0–27

27–43

43–91

91–120

pH influent

7.0

7.0

5.8

5.5

pH reactor

6.4 ± 0.3

6.3 ± 0.2

5.8

5.5

CO2 supply (mL/min)

0.18

0.18

0.36

0.40

  1. The changed parameters are indicated in italic
  2. The pH in the reactor was controlled during phase III and phase IV