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Table 4 Setup for the different phases of the continuous formation of valerate from propionate and methanol to study the effect of pH lowering on n-valerate formation

From: Continuous n-valerate formation from propionate and methanol in an anaerobic chain elongation open-culture bioreactor

Phase conditions Phase I Phase II Phase III Phase IV
Hydraulic retention time (h) 42.3 95.2 95.2 95.2
Phase duration (days) 0–27 27–43 43–91 91–120
pH influent 7.0 7.0 5.8 5.5
pH reactor 6.4 ± 0.3 6.3 ± 0.2 5.8 5.5
CO2 supply (mL/min) 0.18 0.18 0.36 0.40
  1. The changed parameters are indicated in italic
  2. The pH in the reactor was controlled during phase III and phase IV