Fig. 1

Raman microspectroscopy and imaging of single Euglena gracilis cells under anaerobic condition. A Space-resolved Raman spectra measured at polysaccharide-rich region (a), and ester-rich region (b) from a cell at 24 h. B Optical images of single E. gracilis cells (c), time-resolved univariate Raman images of polysaccharides (d), and esters (e). Scale bar in each optical image measures 10 μm and measured points are indicated using alphabets