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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: A novel thermostable chitinolytic machinery of Streptomyces sp. F-3 consisting of chitinases with different action modes

Fig. 2

Effect of temperature and pH on chitinases of Streptomyces sp. F-3. a The optimum reaction temperature of the chitinases. Chitinase activity was measured with colloidal chitin at temperatures ranging from 30 to 80 °C at pH 5.0 for 30 min. b The temperature stability of the chitinases. The temperature stability was determined by measuring the remaining activity after incubation at various temperatures at pH 5.0 for 30 min without substrate. c The optimum reaction pH. Chitinase activity was determined at various pH values (pH 3–8, 50 mM Na2HPO4-citric acid buffer; pH 9–12, 50 mM Na2HPO4–NaOH buffer) at 60 °C for 30 min. d The pH stability of the chitinases. The pH stability was determined by measuring the residual activity after 30 min incubation at various pH values at 20 °C without substrate. Error bars are given as means and standard deviations

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