Fig. 3

FACE analysis of hydrolysis products by chitinases from various CHOSs. The reaction mixture (100 μL) containing 60 μL of 1 mg/mL substrate was incubated with purified enzyme (40 μL, 0.01 nmol/mL) at 60 °C. The reaction mixture was analyzed at the indicated times. Standard CHOSs from GlcNAc (G1) to GlcNAc6 (G6). a, f, k, p, u For SsChi18A; b, g, l, q, v for SsChi18B; c, h, m, r, w for SsChi18C; d, i, n, s, x for SsChi19A; and e, j, o, t, y for SsGH20A