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Table 1 Substrate specificity of chitinases from Streptomyces sp. F-3

From: A novel thermostable chitinolytic machinery of Streptomyces sp. F-3 consisting of chitinases with different action modes

Substrate Specific activity (U/μmol)a
SsChi18A SsChi18B SsChi18C SsChi19A SsGH20A
Colloidal chitin 54.33 ± 0.55 34.74 ± 2.72 28.61 ± 2.16 5.09 ± 0.83 1.97 ± 0.18
Chitin powder 4.81 ± 0.14 1.75 ± 0.10 1.50 ± 0.15 2.02 ± 0.26 0.97 ± 0.57
Chitosanb 0.41 ± 0.06 0.19 ± 0.08 0.28 ± 0.02 0.22 ± 0.07 0.09 ± 0.07
CMC ND ND ND 0.090 ± 0.05 ND
(GlcNAc)6 3.9 ± 0.2 × 103 10.8 ± 0.5 × 103 11.6 ± 0.8 × 103 6.9 ± 0.6 × 103 89.4 ± 1.0 × 103
(GlcNAc)5 4.6 ± 0.1 × 103 7.7 ± 0.2 × 103 8.9 ± 0.6 × 103 6.5 ± 0.5 × 103 63.8 ± 0.9 × 103
(GlcNAc)4 4.8 ± 0.2 × 103 7.0 ± 0.1 × 103 15.1 ± 0.5 × 103 9.9 ± 0.8 × 103 93.6 ± 1.6 × 103
(GlcNAc)3 6.3 ± 0.2 × 103 8.0 ± 0.3 × 103 5.3 ± 0.2 × 103 4.0 ± 0.3 × 103 76.3 ± 1.0 × 103
(GlcNAc)2 ND ND ND ND 68.7 ± 0.6 × 103
  1. ND, not detectable
  2. aSpecific activity was obtained by detecting the generation of the reducing sugar after the reaction mixtures were incubated at 60 °C for 30 min. In the reaction system, the concentration of the polysaccharides was 10 mg/ml, and the concentration of the oligosaccharide substrates was 1 mg/ml. Results are presented as the mean ± standard error (n = 3)
  3. bChitosan, with a degree of acetylation of less than 10%