Biotechnology for Biofuels and Bioproducts

Table 1 Substrate specificity of chitinases from Streptomyces sp. F-3

From: A novel thermostable chitinolytic machinery of Streptomyces sp. F-3 consisting of chitinases with different action modes

Substrate	Specific activity (U/μmol)^a
Substrate	SsChi18A	SsChi18B	SsChi18C	SsChi19A	SsGH20A
Colloidal chitin	54.33 ± 0.55	34.74 ± 2.72	28.61 ± 2.16	5.09 ± 0.83	1.97 ± 0.18
Chitin powder	4.81 ± 0.14	1.75 ± 0.10	1.50 ± 0.15	2.02 ± 0.26	0.97 ± 0.57
Chitosan^b	0.41 ± 0.06	0.19 ± 0.08	0.28 ± 0.02	0.22 ± 0.07	0.09 ± 0.07
CMC	ND	ND	ND	0.090 ± 0.05	ND
(GlcNAc)6	3.9 ± 0.2 × 10³	10.8 ± 0.5 × 10³	11.6 ± 0.8 × 10³	6.9 ± 0.6 × 10³	89.4 ± 1.0 × 10³
(GlcNAc)5	4.6 ± 0.1 × 10³	7.7 ± 0.2 × 10³	8.9 ± 0.6 × 10³	6.5 ± 0.5 × 10³	63.8 ± 0.9 × 10³
(GlcNAc)4	4.8 ± 0.2 × 10³	7.0 ± 0.1 × 10³	15.1 ± 0.5 × 10³	9.9 ± 0.8 × 10³	93.6 ± 1.6 × 10³
(GlcNAc)3	6.3 ± 0.2 × 10³	8.0 ± 0.3 × 10³	5.3 ± 0.2 × 10³	4.0 ± 0.3 × 10³	76.3 ± 1.0 × 10³
(GlcNAc)2	ND	ND	ND	ND	68.7 ± 0.6 × 10³

ND, not detectable
^aSpecific activity was obtained by detecting the generation of the reducing sugar after the reaction mixtures were incubated at 60 °C for 30 min. In the reaction system, the concentration of the polysaccharides was 10 mg/ml, and the concentration of the oligosaccharide substrates was 1 mg/ml. Results are presented as the mean ± standard error (n = 3)
^bChitosan, with a degree of acetylation of less than 10%

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