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Fig. 8 | Biotechnology for Biofuels

Fig. 8

From: A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

Fig. 8

MPD analysis enables correlation between morphological parameters and A. niger protein secretion. a 1 × 106 spores/mL of conditional expression mutants and MA70.15 control were inoculated in 20 mL MM with 5% glucose as carbon source and supplemented with various concentrations of Dox. Cultures were grown at 220 RPM, 30 °C, for 72 h. Total secreted protein (mg) was determined from culture supernatants using a Bradford assay, which was normalised to fungal biomass (g dry weight). Error bars report standard deviation from triplicate biological replicates, each consisting of duplicate technical replicates. Pairwise Student‘s t tests were conducted between TC18.1 and TC18.3 relative to MA70.15 control at respective Dox concentrations. p values < 0.05 are depicted with an asterisk. b Scatter plots where average total protein secretion (mg protein/g dry weight) was calculated for each strain/Dox concentration and plotted as a function of average pellet diameter, aspect ratio, MN, and percentage of pelleted morphologies present in the culture. Linear lines of best fit (dotted blue lines) are depicted, with R2 values shown for each correlation

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