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Table 3 Comparison of fermentation performance of improved strains with mutated LpXI

From: Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Strain LpXI Cxyl36 (g/L/h)a Cxyl72 (g/L/h)b Yeth72 (g/g-input sugars)c
SS81 Wild-type 0.36 ± 0.08 0.30 ± 0.08 0.39 ± 0.03
SS82 T63I/A121A 0.68 ± 0.06 0.47 ± 0.01 0.44 ± 0.01
SS92 V162A/T273T/N303T 0.76 ± 0.04 0.48 ± 0.02 0.44 ± 0.01
SS104 V162A 0.59 ± 0.05 0.46 ± 0.01 0.43 ± 0.01
SS105 N303T 0.32 ± 0.06 0.25 ± 0.02 0.40 ± 0.01
SS120 T63I/V162A 0.75 ± 0.05 0.49 ± 0.00 0.44 ± 0.01
  1. aCxyl36: d-xylose consumption rate in 36 h
  2. bCxyl72: d-xylose consumption rate in 72 h
  3. cYeth72: ethanol yield in 72 h per input sugars
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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