Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Table 3 Comparison of fermentation performance of improved strains with mutated LpXI

Strain	LpXI	C_xyl36 (g/L/h)^a	C_xyl72 (g/L/h)^b	Y_eth72 (g/g-input sugars)^c
SS81	Wild-type	0.36 ± 0.08	0.30 ± 0.08	0.39 ± 0.03
SS82	T63I/A121A	0.68 ± 0.06	0.47 ± 0.01	0.44 ± 0.01
SS92	V162A/T273T/N303T	0.76 ± 0.04	0.48 ± 0.02	0.44 ± 0.01
SS104	V162A	0.59 ± 0.05	0.46 ± 0.01	0.43 ± 0.01
SS105	N303T	0.32 ± 0.06	0.25 ± 0.02	0.40 ± 0.01
SS120	T63I/V162A	0.75 ± 0.05	0.49 ± 0.00	0.44 ± 0.01

ISSN: 2731-3654