Biotechnology for Biofuels and Bioproducts

Table 4 Kinetic properties of wild-type and mutated LpXIs

From: Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Strain	LpXI	V ^a_max	K_m (mM)
SS81	Wild-type	0.064 ± 0.002	41.8 ± 0.8
SS82	T63I/A121A	0.092 ± 0.001	29.3 ± 2.6
SS92	V162A/T273T/N303T	0.066 ± 0.001	28.4 ± 2.3
SS104	V162A	0.063 ± 0.002	29.5 ± 2.2
SS105	N303T	0.030 ± 0.001	19.1 ± 0.6
SS120	T63I/V162A	0.107 ± 0.002	37.1 ± 2.3

The mean and standard deviations of biological triplicates are indicated
^aμmol mg protein⁻¹ min⁻¹

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ISSN: 2731-3654

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