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Table 4 Kinetic properties of wild-type and mutated LpXIs

From: Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae

Strain LpXI V max a Km (mM)
SS81 Wild-type 0.064 ± 0.002 41.8 ± 0.8
SS82 T63I/A121A 0.092 ± 0.001 29.3 ± 2.6
SS92 V162A/T273T/N303T 0.066 ± 0.001 28.4 ± 2.3
SS104 V162A 0.063 ± 0.002 29.5 ± 2.2
SS105 N303T 0.030 ± 0.001 19.1 ± 0.6
SS120 T63I/V162A 0.107 ± 0.002 37.1 ± 2.3
  1. The mean and standard deviations of biological triplicates are indicated
  2. aμmol mg protein−1 min−1