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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: High-efficiency nuclear transformation of the microalgae Nannochloropsis oceanica using Tn5 Transposome for the generation of altered lipid accumulation phenotypes

Fig. 2

Nannochloropsis oceanica mutant originate heterogeneous population with different altered lipid accumulation phenotypes. Flow cytometry analysis in a. Dot plot of N. oceanica mutant and wild type strains selecting low, intermediate and high populations according Bodipy 505/515 fluorescence. b Fluorescence graph of Bodipy 505/515 in Low, intermediate and high populations in both mutant and wild type strains. c Histograms of Bodipy 505/515 fluorescence in wild type (WT), high lipid (HL) and low lipid (LL) cells in day 9 of the growth curve (stationary phase). The Y axis shows the number of cells and X axis fluorescence intensity (Bodipy 505/515). Blue curve: LL cells; Red curve: HL cells; and Gray curve: WT strain. d Fluorescence quantification graph of fluorophore Bodipy 505/515. The Y axis shows fluorescence units with their respective standard deviation (SD). All populations had the same number of cells analyzed (n = 50.000). Error bars in the figure represent four replicates

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