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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: High-efficiency nuclear transformation of the microalgae Nannochloropsis oceanica using Tn5 Transposome for the generation of altered lipid accumulation phenotypes

Fig. 3

Single-cell LL and HL clones showed altered lipid accumulation. a Flow diagram of single-cell sorting and validation. b PCR validation of transposon insertion into the genome of mutant clones. The figure shows PCR products (374 bp) of the Zeocin resistance gene. (−) Negative PCR control and (+) positive PCR control with pMOD-Zeo plasmid. Also, numbers from 1 to 25 correspond to mutant clones with high-lipid phenotype (upper gel) and low lipid phenotype (lower gel). Red arrow indicates the size of 374 bp of the amplified product. c Validation of single-cell clones by flow cytometry, showing Bodipy 505/515 fluorescence intensity of clones. All populations had the same number of cells analyzed (n = 50.000) One-way ANOVA and unpaired t test were used (p < 0.05)

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