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Table 2 Effect of cellulase recycling on the overall glucose yields and total amounts of produced glucose (mean ± standard deviation) from the enzymatic hydrolysis of hydrothermally-pretreated EFBs

From: Cellulase recycling in high-solids enzymatic hydrolysis of pretreated empty fruit bunches

Mode of  the two-round enzymatic hydrolysis

Hydrolysis round

Cellulase loading (FPU/g glucan)

Glucose yield (% of theoretical max.)

Total amount of glucose produced (g)c

Separate hydrolysis without cellulase recycling

R1a

20

78.2 ± 0.1

3.70 ± 0.00

R2b

20

78.2 ± 0.1

Cellulase recycling

R1a

40

98.7 ± 0.8

2.79 ± 0.15

R2b

0

19.3 ± 5.7

Cellulase recycling with the additional treatments

R1a

40

98.7 ± 0.8

3.94 ± 0.06

R2b

0

68.0 ± 1.8

  1. aThe first round of enzymatic hydrolysis
  2. bThe second round of enzymatic hydrolysis
  3. cThe overall glucose produced from 4 g of pretreated EFBs at the first round and 4 g of pretreated EFBs at the second round