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Table 3 Fatty acid profiles of R. toruloides during early exponential phase and lipid accumulation phase following batch cultivation in medium containing either glucose or xylose (presented as percentage of total identified, average ± standard deviation)

From: Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production

 

t0

t1

t2

t3

 

Glc = Xyl

Xylose

Glucose

Xylose

Glucose

Xylose

Glucose

C14:0

0.98 ± 0.25

1.03 ± 0.26

0.91 ± 0.03

1.23 ± 0.09

1.25 ± 0.05

1.28 ± 0.04

1.1 ± 0.07

C16:0

14.35 ± 1.9

17.91 ± 2.5

17.16 ± 0.01

26.63 ± 0.22

25.76 ± 0.24

26.73 ± 0.26

25.55 ± 0.3

C16:1

1.39 ± 0.28

0.96 ± 0.1

0.51 ± 0.02

0.98 ± 0.05

0.53 ± 0.01

1.00 ± 0.04

0.57 ± 0.05

C17:0

n.d.

0.76 ± 0.13

n.d.

n.d.

n.d.

n.d.

n.d.

C17:1

0.75 ± 0.18

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

C18:0

3.84 ± 0.44

5.55 ± 0.57

7.63 ± 0.5

8.11 ± 0.28

9.31 ± 0.19

7.73 ± 0.22

7.83 ± 0.56

C18:1 (n − 9)

66.66 ± 2.28

46.27 ± 3.05

36.99 ± 1.3

45.26 ± 0.5

47.23 ± 0.61

45.07 ± 0.7

47.25 ± 1.06

C18:2 (n − 6)

6.62 ± 0.33

20.36 ± 4.16

23.56 ± 0.71

13.51 ± 0.69

11.58 ± 0.5

13.83 ± 0.57

13.06 ± 0.68

C18:3 (n − 3)

3.38 ± 0.86

5.74 ± 1.9

11.25 ± 0.98

2.78 ± 0.15

2.94 ± 0.22

2.99 ± 0.19

3.01 ± 0.25

C24:0

0.9 ± 0.01

1.15 ± 0.13

0.66 ± 0.05

0.73 ± 0.05

0.76 ± 0.02

0.67 ± 0.05

0.91 ± 0.13

Avg length

17.4891

17.5942

17.4104

17.3038

17.3546

17.3084

17.3586

Avg desaturation

0.9218

1.0517

1.1837

0.816

0.7974

0.827

0.8297

  1. Samples were collected at the beginning of cultivation (t0) as well as during early exponential phase (t1), late exponential phase (t2) and lipid production phase (t3). Polyunsaturated fatty acids were counted for the number of unsaturations according to [18]
  2. n.d. not detected