The extracellular endo-β-1,4-xylanase with multidomain from the extreme thermophile Caldicellulosiruptor lactoaceticus is specific for insoluble xylan degradation

Table 3 Comparison of properties between C. lactoaceticus Xyn10B truncated variants and other thermophilic xylanases

Xylanases from microorganism	Domain^a	Mw (kDa)^b	Topt (°C)	pHopt	Specific activity (IU/μmol)		References
Xylanases from microorganism	Domain^a	Mw (kDa)^b	Topt (°C)	pHopt	BWX	OSX	References
C. lactoaceticus Xyn10B-TM1	A1-A2-A3-B	91.0	65	6.0	944.3 ± 16.5	5371.5 ± 281.1	This study
Xyn10B-TM2	A3-B	57.5	65	6.0	648.9 ± 10.3	3720.4 ± 217.8	This study
Xyn10B-TM3	A3-B-C1	73.6	65	6.5	457.5 ± 5.5	1106.1 ± 65.0	This study
Xyn10B-TM4	B	39.3	65	6.5	284.0 ± 15.5	225.4 ± 31.8	This study
Thermoanaerobacterium aotearoense SCUT27 XynAΔSLH	A1-A2-B-C1-C2	113	80	6.5	42,917.4 ± 361.6	24159.4 ± 180.8	[20]
T. saccharolyticum NTOU1 XynA	A1-A2-B-C1-C2-D1-D2-D3	154	72	5.5	66836.0 ± 2464	22022.0 ± 1078.0	[34]
Alkaline wastewater sludge Xyn-b39	A1-A2-B-C1-D1-D2-D3	160	60	7.0	6588.8 ± 384	2464.0 ± 272	[35]
Thermotoga thermarum Xyn10A	A1-A2-A3-B-C1-C2	131	95	7.0	19101.11	NA	[36]
T. saccharolyticum B6A-RI XynA	A1-A2-B-C1-C2	130	70	5.5	NA	29562	[37]
Bacillus sp. BP-23 xylanase C	A1-A2-B-C1-C2	120.6	45	5.0	NA	12.06	[38]
T. neapolitana XynA	A1-A2-B-C1-C2	116	102	5.5	NA	12910.8	[39]

Xyn Xylanase, Mw molecular weight, Topt optimum temperature, pHopt optimum pH, BWX beech wood xylan, OSX oat spelt xylan, NA not available
^aA1, A2, A3, B, C1, C2, D1, D2 represents the N and/or C-terminal domains, A1, A2, A3: N-terminal family 22 CBM; B: catalytic domain; C1, C2: C-terminal family 9 CBM; D1, D2, D3: C-terminal SLH
^bValue by SDS-PAGE

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