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Table 1 Modular organization of Xyl-Doc enzymes and putative functions

From: The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-α-l-arabinofuranosidases with complementary modes of action

Locusa Xyl-Doc enzymeb Modular structurec NCBI-protein ID Putative functiond
Ccel_1229 GH4316-1229 S-GH4316-CBM6-DOC ACL75585 Xylanase/α-l-arabinofuranosidase
Ccel_1230 GH10-1230 S-GH10-UNK-CBM6-DOC ACL75586 Xylanase
Ccel_1231 GH4329-1231 S-GH4329-CBM6-DOC ACL75587 Xylanase/xylosidase/α-l-arabinofuranosidase
Ccel_1232 CE1-1232 S-CE1-CBM6-DOC ACL75588 Feruloyl esterase
Ccel_1233 GH4310-1233 S-GH4310-X19-CBM6-DOC ACL75589 Xylosidase/α-l-arabinofuranosidase
Ccel_1234 GH62-1234 S-GH62-CBM6-DOC ACL75590 α-l-Arabinofuranosidase
Ccel_1235 GH4329-1235 S-GH4329-CBM6-DOC ACL75591 Xylanase/α-l-arabinofuranosidase
Ccel_1236 UNK-1236 S-COG3533-CBM22-UNK- DOC ACL75592 Unknown
Ccel_1237 GH27-1237 S-GH27-UNK-CBM6-DOC ACL75593 α-Galactosidase
Ccel_1238 GH59-1238 S-GH59-UNK-CBM6-DOC ACL75594 α-Galactosidase
Ccel_1239 GH2-1239 S-UNK-GH2-UNK-CBM6-DOC ACL75595 β-Galactosidase
Ccel_1240 GH62-CE6-1240 S-GH62-CBM6-DOC-CE6 ACL75596 α-l-Arabinofuranosidase/acetyl-xylan esterase
Ccel_1241 GH95-1241 S-UNK-GH95-CBM32-CBM6-DOC ACL75597 Fucosidase
Ccel_1242 GH30-1242 S-GH30-UNK-CBM6-DOC ACL75598 Glucuronoxylanase
  1. For GH, CE, and CBM, the family is given by an associated number according to the CAZy database [22]. For GH43 subfamily is mentioned by a subscript number [23]. UNK: module of unknown function. X19: additional module of unknown function found associated with GH43 catalytic module in some proteins. COG3533: uncharacterized conserved module. DOC: dockerin module
  2. S signal sequence, GH glycoside hydrolase, CE carbohydrate esterase, CBM carbohydrate-binding module
  3. axyl-doc gene cluster (loci Ccel_1229 to Ccel_1242)
  4. bNotation of each Xyl-Doc enzymes according to its DNA locus and GH family
  5. cModular organization
  6. dPutative function [19]