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Table 3 Specific activities of the Xyl-Doc α-l-ABFs on various substrates and kinetic parameters on WAXY-I

From: The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-α-l-arabinofuranosidases with complementary modes of action

  WAXY-RSa WAXY-I OSX Sugar-beet arabinan
GH4316-1229 0.191 ± 0.005 0.028 ± 0.007b
kcat and Km: ND
0.085 ± 0.056b NA
GH4310-1233 453.7 ± 13 386 ± 37
kcat 16.6 ± 0.6c
Km 24.8 ± 0.1c
71 ± 1b 166 ± 2.8b
GH62-1234 4.29 ± 0.14 3.38 ± 0.56b
kcat = 0.124 ± 0.002c
Km = 3.735 ± 0.072c
4.7 ± 0.1b 1.3 ± 0.06b
GH62-CE6-1240 3 ± 0.13 2.2 ± 0.14b
kcat = 0.114 ± 0.001c
Km = 4.066 ± 0.068c
3.4 ± 0.1b 0.78 ± 0.12b
  1. WAXY-I insoluble wheat-flour arabinoxylan insoluble, OSX oat spelt xylan. ND means not determined due to insufficient activity or lack of detectable activity
  2. NA not active
  3. aSpecific activities from Table 2
  4. bSpecific activity in IU/µmol. The data show the mean and standard deviation of 6 independent experiments
  5. ckcat is given in s−1, Km in g/L