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Table 3 Specific activities of the Xyl-Doc α-l-ABFs on various substrates and kinetic parameters on WAXY-I

From: The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-α-l-arabinofuranosidases with complementary modes of action

 

WAXY-RSa

WAXY-I

OSX

Sugar-beet arabinan

GH4316-1229

0.191 ± 0.005

0.028 ± 0.007b

kcat and Km: ND

0.085 ± 0.056b

NA

GH4310-1233

453.7 ± 13

386 ± 37

kcat 16.6 ± 0.6c

Km 24.8 ± 0.1c

71 ± 1b

166 ± 2.8b

GH62-1234

4.29 ± 0.14

3.38 ± 0.56b

kcat = 0.124 ± 0.002c

Km = 3.735 ± 0.072c

4.7 ± 0.1b

1.3 ± 0.06b

GH62-CE6-1240

3 ± 0.13

2.2 ± 0.14b

kcat = 0.114 ± 0.001c

Km = 4.066 ± 0.068c

3.4 ± 0.1b

0.78 ± 0.12b

  1. WAXY-I insoluble wheat-flour arabinoxylan insoluble, OSX oat spelt xylan. ND means not determined due to insufficient activity or lack of detectable activity
  2. NA not active
  3. aSpecific activities from Table 2
  4. bSpecific activity in IU/µmol. The data show the mean and standard deviation of 6 independent experiments
  5. ckcat is given in s−1, Km in g/L