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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

Fig. 4

Determination of putative catalytic sites of the CscA. a SDS-PAGE analysis of the purified unmutated CscA enzyme and Asp37, Asp161, and Glu215 mutant enzymes. Lane M: premixed protein marker (low); lane 2: purified CscA enzyme; lane 3: D37A; lane 4: D161A; lane 5: E215A. b The secondary structure and putative catalytic sites of the CscA, superposed to A. awamori exo-inulinase (PDB entry 1Y9G). c Inulinase activity assay of mutant inulinase hydrolases

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