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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

Fig. 5

Genetic analysis of the role of the cscA gene in inulin metabolism of B. amyloliquefaciens NB. a Illustration of putative cscA disruption process. b PCR confirmation of the cscA disruption. Lane M: DL15,000 marker; lane 1: the control strain NB; lanes 2–7: cscA deletion mutants NBΔC. c Image of growth array of cscA deletion mutants using inulin or inulin extract as the sole carbon source. d Growth assay of cscA deletion variant NBΔC and complementation transformant NBΔC-C supplemented with inulin or sucrose. e Inulinase activity assay of complementation transformant NBΔC-C (lane 2) and the cscA gene overexpressing strain, NB-C (lane 3). f Time-dependent profile of γ-PGA fermentation from raw inulin extract by strain NB-C. The filled symbols represent the fermentation process of wild-type strain NB and empty symbols represent the fermentation process of strain NB-C

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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