Skip to main content

Table 1 Strains and plasmids used in this study

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

Strains or plasmids Relevant properties Source
 E. coli DH5α F, φ80dlacZΔM1, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk, mk+), phoA, supE44, λthi-1, gyrA96, relA1 This lab
 E. coli GM2163 F, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (Strr) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 mcrA This lab
 E. coli BL21(DE3) F-, ompT hsdSB (rB mB) gal dcm (DE3) This lab
 B. amyloliquefaciens NB NX-2S derivate, BamHI::PHpaII-pgsR This lab
 B. amyloliquefaciens NBΔC NBΔCscA This study
 B. amyloliquefaciens NBΔC-C NBΔCscA containing pNX-CscA This study
 B. amyloliquefaciens NB-C NB containing pNX-CscA This study
 pNX01 E. coli and B. amyloliquefaciens expression vector; AmpR, CmR This lab
 pNX01-CscA pNX01 carrying CscA gene from B. amyloliquefaciens NB used for overexpression of the CscA gene This study
 pDR-pheS* E. coli and B. amyloliquefaciens knockout vector; AmpR, Spec; pDR with PP43-pheS* cassette This lab
 pDR-pheS*-CscA’ pDR-pheS* carrying the homology arm of CscA gene used for deletion of the CscA gene This study