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Strains
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E. coli DH5α
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F−, φ80dlacZΔM1, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk−, mk+), phoA, supE44, λ−thi-1, gyrA96, relA1
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This lab
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E. coli GM2163
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F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (Strr) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 mcrA
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This lab
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E. coli BL21(DE3)
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F-, ompT hsdSB (rB− mB−) gal dcm (DE3)
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This lab
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B. amyloliquefaciens NB
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NX-2S derivate, BamHI::PHpaII-pgsR
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This lab
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B. amyloliquefaciens NBΔC
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NBΔCscA
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This study
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B. amyloliquefaciens NBΔC-C
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NBΔCscA containing pNX-CscA
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This study
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B. amyloliquefaciens NB-C
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NB containing pNX-CscA
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This study
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Plasmids
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pNX01
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E. coli and B. amyloliquefaciens expression vector; AmpR, CmR
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This lab
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pNX01-CscA
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pNX01 carrying CscA gene from B. amyloliquefaciens NB used for overexpression of the CscA gene
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This study
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pDR-pheS*
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E. coli and B. amyloliquefaciens knockout vector; AmpR, Spec; pDR with PP43-pheS* cassette
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This lab
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pDR-pheS*-CscA’
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pDR-pheS* carrying the homology arm of CscA gene used for deletion of the CscA gene
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This study
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