Table 1 Strains and plasmids used in this study
Strains or plasmids | Relevant properties | Source |
|---|---|---|
Strains | ||
E. coli DH5α | F−, φ80dlacZΔM1, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk−, mk+), phoA, supE44, λ−thi-1, gyrA96, relA1 | This lab |
E. coli GM2163 | F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (Strr) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 mcrA | This lab |
E. coli BL21(DE3) | F-, ompT hsdSB (rB− mB−) gal dcm (DE3) | This lab |
B. amyloliquefaciens NB | NX-2S derivate, BamHI::PHpaII-pgsR | This lab |
B. amyloliquefaciens NBΔC | NBΔCscA | This study |
B. amyloliquefaciens NBΔC-C | NBΔCscA containing pNX-CscA | This study |
B. amyloliquefaciens NB-C | NB containing pNX-CscA | This study |
Plasmids | ||
pNX01 | E. coli and B. amyloliquefaciens expression vector; AmpR, CmR | This lab |
pNX01-CscA | pNX01 carrying CscA gene from B. amyloliquefaciens NB used for overexpression of the CscA gene | This study |
pDR-pheS* | E. coli and B. amyloliquefaciens knockout vector; AmpR, Spec; pDR with PP43-pheS* cassette | This lab |
pDR-pheS*-CscA’ | pDR-pheS* carrying the homology arm of CscA gene used for deletion of the CscA gene | This study |