Table 2 Primers and their sequences used for PCR in this study
Primers | Sequences (5′–3′) |
|---|---|
pETSACC-F | CAGCAAATGGGTCGCGGATCCATGGCACCAAAATGGCCC |
pETSACC-R | GTGGTGGTGGTGGTGCTCGAGCTAAGGATGAATCGAACCGAGAT |
pETCSCA-F | CAGCAAATGGGTCGCGGATCCATGGATAGAATTCAGCAGGCGG |
pETCSCA-R | GTGGTGGTGGTGGTGCTCGAGTCACTTTTTTTCCAAATGTCCTTTAC |
D37A-1 | CTTAAACTGAATAAGTCCGTTCGGCGCATTGATCCAATTCGCCCGGG |
D37A-2 | TTCAAAGAAATCAGGACACGCCCACATATAGCCGAGATG |
D161A-1 | TTCAAAGAAATCAGGACACGCCCACATATAGCCGAGATG |
D161A-2 | GGTCATCTCGGCTATATGTGGGCGTGTCCTGATTTCTTTGAATTA |
E215A-1 | GCGATGCTTCCACACTTTCGGCGCGCGAAAATGGCGGGAGCT |
E215A-1 | AGCTCCCGCCATTTTCGCGCGCCGAAAGTGTGGAAGCAT |
pNXCSCA-F | AAAAGGAGCGATTTAGGTACCATGGATAGAATTCAGCAGGCGG |
pNXCSCA-R | TGTTTTTTTATTACCCATATGTCACTTTTTTTCCAAATGTCCTTTAC |
pDRCSCA’-F | TAGATAGCGCATGCTGAATTCCCGCCGGAAGACAGCTCC |
pDRCSCA’-R | ACATACTTTAAAAATCTCGAGCGCACTCTTCTCACACCGTCT |
CSCAOUT-F | CCATGACCGAGGATTTGCGGACAATG |
CSCAOUT-R | TGGACCGCTCCTGCAGTACAACGTCT |