|
Metal ions
|
Relative activity (%)
|
|---|
|
1 mM
|
10 mM
|
|---|
|
Nonea
|
100 ± 1
|
100 ± 2
|
|
EDTAb
|
99 ± 1
|
97 ± 1
|
|
ZnCl2
|
83 ± 1
|
76 ± 1
|
|
MnCl2
|
125 ± 1
|
131 ± 2
|
|
CaCl2
|
114 ± 1
|
117 ± 1
|
|
CoCl2
|
92 ± 1
|
84 ± 1
|
|
KCl
|
109 ± 2
|
115 ± 2
|
|
CuCl2
|
43 ± 2
|
–
|
|
AlCl3
|
102 ± 1
|
97 ± 2
|
|
NaCl
|
97 ± 1
|
92 ± 1
|
- The inulinase activity of EDTA-treated CscA was assayed in PBS buffer (1/15 M, pH 7.5) at 55 °C for 20 min after incubating of 1 mM or 10 mM various metal ions. Each value represents the mean of triplicate measurements and varied from the mean by not more than 10%
- aThe activity of purified CscA enzyme without EDTA treatment and metal ions addition was set as 100%
- bThe activity of purified CscA enzyme after EDTA treatment but without metal ions addition
