Metal ions | Relative activity (%) |
|---|
1 mM | 10 mM |
|---|
Nonea | 100 ± 1 | 100 ± 2 |
EDTAb | 99 ± 1 | 97 ± 1 |
ZnCl2 | 83 ± 1 | 76 ± 1 |
MnCl2 | 125 ± 1 | 131 ± 2 |
CaCl2 | 114 ± 1 | 117 ± 1 |
CoCl2 | 92 ± 1 | 84 ± 1 |
KCl | 109 ± 2 | 115 ± 2 |
CuCl2 | 43 ± 2 | – |
AlCl3 | 102 ± 1 | 97 ± 2 |
NaCl | 97 ± 1 | 92 ± 1 |
- The inulinase activity of EDTA-treated CscA was assayed in PBS buffer (1/15 M, pH 7.5) at 55 °C for 20 min after incubating of 1 mM or 10 mM various metal ions. Each value represents the mean of triplicate measurements and varied from the mean by not more than 10%
- aThe activity of purified CscA enzyme without EDTA treatment and metal ions addition was set as 100%
- bThe activity of purified CscA enzyme after EDTA treatment but without metal ions addition
