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Table 4 Substrate specificity of purified CscA of B. amyloliquefaciens NB

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

Substratea

Vmax (μmol min−1 mg−1)

Km (mM)

kcat/Km (min−1 mM−1)

Inulin

(9.92 ± 0.31) × 102

8.35 ± 0.04

(6.93 ± 0.27) × 103

Sucrose

(1.76 ± 0.05) × 103

4.33 ± 0.03

(2.39 ± 0.12) × 104

Raffinose

(7.24 ± 0.11) × 102

12.25 ± 0.07

(3.45 ± 0.08) × 103

Stachyose

(6.87 ± 0.29) × 102

19.08 ± 0.09

(2.19 ± 0.04) × 103

Fructooligosaccharide

(9.68 ± 0.32) × 102

10.22 ± 0.06

(5.84 ± 0.05) × 103

Soluble starch

  1. aAll substrates were dissolved or suspended in PBS buffer