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Table 4 Substrate specificity of purified CscA of B. amyloliquefaciens NB

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

Substratea Vmax (μmol min−1 mg−1) Km (mM) kcat/Km (min−1 mM−1)
Inulin (9.92 ± 0.31) × 102 8.35 ± 0.04 (6.93 ± 0.27) × 103
Sucrose (1.76 ± 0.05) × 103 4.33 ± 0.03 (2.39 ± 0.12) × 104
Raffinose (7.24 ± 0.11) × 102 12.25 ± 0.07 (3.45 ± 0.08) × 103
Stachyose (6.87 ± 0.29) × 102 19.08 ± 0.09 (2.19 ± 0.04) × 103
Fructooligosaccharide (9.68 ± 0.32) × 102 10.22 ± 0.06 (5.84 ± 0.05) × 103
Soluble starch
  1. aAll substrates were dissolved or suspended in PBS buffer