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Table 5 Kinetic parameters of the wild-type and the CscA mutations

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

 

Kcat (min−1)

Km (mM)

Kcat/Km (min−1 mM−1)

Relative enzyme efficiency (%)

Wild-type

57,800 ± 0.38

8.35 ± 0.04

6930 ± 0.27

100

D37A

3.4 ± 0.05

8.29 ± 0.05

4.1 ± 0.12

0.06

D161A

2.4 ± 0.11

8.32 ± 0.08

3.1 ± 0.08

0.04

E215A

5800 ± 0.09

9.74 ± 0.12

595 ± 0.14

8.6

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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