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Table 5 Kinetic parameters of the wild-type and the CscA mutations

From: Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(γ-glutamic acid)

  Kcat (min−1) Km (mM) Kcat/Km (min−1 mM−1) Relative enzyme efficiency (%)
Wild-type 57,800 ± 0.38 8.35 ± 0.04 6930 ± 0.27 100
D37A 3.4 ± 0.05 8.29 ± 0.05 4.1 ± 0.12 0.06
D161A 2.4 ± 0.11 8.32 ± 0.08 3.1 ± 0.08 0.04
E215A 5800 ± 0.09 9.74 ± 0.12 595 ± 0.14 8.6