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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Improving isobutanol production with the yeast Saccharomyces cerevisiae by successively blocking competing metabolic pathways as well as ethanol and glycerol formation

Fig. 7

Reduction of ethanol, glycerol and isobutyric acid biosyntheses increases isobutanol production. Fermentation experiments were performed aerobically at 30 °C in shake flasks in selective SCD medium without valine containing 40 g/L glucose. Pre-cultures carrying the episomal 2µ-plasmid IsoV100 were grown aerobically in fermentation medium, harvested at an OD600 ≤ 3 and inoculated in fresh fermentation medium at a final OD600 of 8. Fermentation experiments were performed at least in duplicate. Error bars indicate standard deviation for experiments performed twice and standard error for experiments performed at least three times, respectively. a Glucose consumption after successively blocking of competing isobutanol pathways by deletion of key enzymes of biosynthesis pathways: CEN.PK113-7D (wt); JWY04 (∆ilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1); JWY16 (∆ilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1); JWY17 (∆ilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd1); JWY18 (∆ilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd2); JWY19 (∆ilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd1; Δgpd2); JWY23 (∆ilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd1; Δgpd2; Δald6). b Isobutanol production. c Glycerol production. d Ethanol production

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