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Table 3 Yeast strains created in this work, originating from CEN.PK113-7D

From: Improving isobutanol production with the yeast Saccharomyces cerevisiae by successively blocking competing metabolic pathways as well as ethanol and glycerol formation

Strain

Modifications

JWY0

Δilv2

JWY01

Δilv2; Δbdh1; Δbdh2

JWY02

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9

JWY03

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31

JWY04

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1

JWY05

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δbat1

JWY06

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δbat2

JWY07

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δbat1; Δbat2

JWY12

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δpdc1::MTH1; Δpdc5

JWY13

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δpdc1; Δpdc5; Δmth1(+169; +393)

JWY14

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δpdc1

JWY15

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δpdc5

JWY16

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1

JWY17

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd1

JWY18

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd2

JWY19

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd1; Δgpd2

JWY23

Δilv2; Δbdh1; Δbdh2; Δleu4; Δleu9; Δecm31; Δilv1; Δadh1; Δgpd1; Δgpd2; Δald6