Fig. 1

Growth of C. necator H16 on 3-HP together with fructose (a) and on 3-HP alone (b). a 24 h end point growth of C. necator H16 following cultivation in F-MM containing 0.4% (22 mM) fructose, and supplemented with increasing concentrations of 3-HP: 0 mM, 8 mM, 16 mM, 32 mM, 64 mM, 128 mM and 256 mM. Growth was determined in a BioLector microbioreactor system by correlating the measured scattered light values to an OD₆₀₀ calibration curve. Error bars represent the standard deviation of the mean for three independent experiments. **** (p < 0.0001), *** (p = 0.0002), ** (p = 0.0041), * (p = 0.0479). b Growth of C. necator H16 with 50 mM 3-HP as the sole source of carbon and energy. Growth was followed by measuring OD₆₀₀ (blue circles), 3-HP concentrations were quantified through HPLC (green triangles). Error bars represent the standard deviation of the mean for three independent experiments