Fig. 3

Growth (left) and 3-HP consumption (right) of C. necator H16 and generated mutant strains. a C. necator H16 mmsA and mcd single knock-out strains. Blue circles represent the H16 wild type; grey triangles, CNCA03 (ΔmmsA1); red squares, CNCA04 (ΔmmsA2); white diamonds, CNCA05 (ΔmmsA3); and brown diamonds, CNCA06 (Δmcd). b C. necator H16 double deletion mutants. Blue circles represent the H16 wild type; red squares, CNCA04; black inverted triangles, CNCA08 (ΔmmsA2ΔmmsA3); grey diamonds, CNCA09 (ΔmmsA2Δmcd); yellow squares, CNCA10 (ΔmmsA2ΔhpdH); turquoise circles, CNCA11 (ΔmmsA3ΔhbdH); and green triangles, CNCA12 (ΔmmsA2ΔmmsA1). c C. necator H16 single, double and triple deletion mutants grown over 240 h. Blue circles represent the H16 wild type; red squares, CNCA04; purple inverted triangles, CNCA07 (Δhpdh); yellow squares, CNCA10; green triangles, CNCA12; and orange diamonds, CNCA13 (ΔmmsA2ΔmmsA1ΔmmsA3). Strains were cultivated in MM supplemented with 50 mM 3-HP as the sole source of carbon and energy. Error bars represent the standard deviation of the mean for three independent experiments