Table 2 The experimental design and results of the central composite design
Run | Factors | Responses | ||
|---|---|---|---|---|
A (KH2PO4, g/L) | B (HAc, g/L) | Y1 (lipid titer, g/L) | Y2 (lipid content, %) | |
1 | 0 (− 1) | 0 (− 1) | 5.4 | 48.3 |
2 | 0.2 (+ 1) | 0 (− 1) | 3.1 | 28.8 |
3 | 0 (− 1) | 15.0 (+ 1) | 5.1 | 57.5 |
4 | 0.2 (+ 1) | 15.0 (+ 1) | 3.5 | 33.2 |
5 | 0 (− 1) | 7.5 (0) | 6.3 | 54.3 |
6 | 0.2 (+ 1) | 7.5 (0) | 4.3 | 32.5 |
7 | 0.1 (0) | 0 (− 1) | 3.8 | 34.8 |
8 | 0.1 (0) | 15.0 (+ 1) | 3.8 | 40.0 |
9 | 0.1 (0) | 7.5 (0) | 4.9 | 37.7 |
10 | 0.1 (0) | 7.5 (0) | 4.7 | 36.8 |
11 | 0.1 (0) | 7.5 (0) | 4.7 | 36.7 |
12 | 0.1 (0) | 7.5 (0) | 4.8 | 37.4 |
13 | 0.1 (0) | 7.5 (0) | 4.7 | 36.8 |