Fig. 4

Evidence of lignin fragment catabolism in fungi. a HPLC results measuring the percentage of compounds remaining in PBS following 72 h of internalization by P. chrysosporium. Percentages were calculated using a 2.5 mM spiked sample. b ASAP-MS results of vanillic acid (m/z = 168.02) and 6C13 vanillic acid (m/z = 174.03) in P. chrysosporium lysate following a time course of internalization from 1 to 10 h. Vanillic acid and 6C13 vanillic acid were measured in separate biological replicates. No counts above background were detected at m/z = 168.02 and m/z = 174.03 in absence of addition of these compounds. c ASAP-MS results of vanillic acid peak intensity in S. cerevisiae following a time course of internalization from 1 to 17 h. A single biological replicate is shown for c